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Invitrogen™ eBioscience™ phospho-AKT 1/2/3 (Ser473) InstantOne™ ELISA Kit

Specifically engineered for accurate measurement of phosphorylated human, mouse, and hamster AKT 1/2/3 in cell lysates. Detection of rat AKT 1/2/3 is expected. The InstantOne™ ELISA kit allows for analysis of samples in approximately one hour.

Merk:  Invitrogen™ 85-86042-11

Overige gegevens : Gewicht : 0.23750kg

 Bekijk meerdere types van dit product via de

Produktcode. 15540777

  • € 512.00 / 96 tests


Geschatte verzenddatum
bij leverancier 27-03-2019
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Waarschuwingen:

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Inclusief: User Manual
InstantOne™ Assay Plate - 96 well (sealed)
Cell Lysis Buffer (5X)
Enhancer Solution
Wash Buffer (10X)
phospho-AKT 1/2/3 (Ser473) Capture Antibody Reagent
phospho-AKT 1/2/3 (Ser473) Detection Antibody Reagent
Positive Control Cell Lysate (lyophilized)
Detection Reagent (TMB Substrate)
Stop Solution
Plate seal

Beschrijving

Beschrijving

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
Specificatie

Specificatie

P31749
ELISA
Colorimetric Microplate Reader
ELISA
RUO
Cell Lysates (20μL)
2-8°C
1 hr 10 min
AKT1
HRP
Biotin
207
Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
96 tests
Cell Lysates
Hamster, Human, Mouse
1 hr 20 min
Human
PKB, Protein Kinase B, RAC-PK-alpha
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