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Invitrogen™ Phospho-EEF2K (Ser500) Polyclonal Antibody
Rabbit Polyclonal Antibody
Brand: Invitrogen™ PA5143691
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Description
The antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human recombinant eEF2K autophosphorylated in the presence of Ca2+ and Calmodulin. This reactivity is removed after lambda phosphatase treatment. In addition, the antibody detects eEF2K after calyculin A treatment of mouse C2C12 cells, but not in control cells.
EEF2K is a highly conserved Serine/Threoning kinase in the calmodulin signaling pathway whose activity is increased in cancers including breast cancer. EEF2K (eukaryotic elongation factor 2 kinase) is a threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Up activation, by a variety of upstream kinases including AMPK or TRPM7, EEF2K phosphorylates the elongation factor EEF2 at a single site. Then, EEF2K renders EEF2 unable to bind ribosomes and inactive - in turn, the rate of protein synthesis is reduced.
Specifications
| Phospho-EEF2K (Ser500) | |
| Polyclonal | |
| Unconjugated | |
| EEF2K | |
| C86191; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; EC 2.7.11.20; eEF-2 kinase; Eef2k; eEF-2K; EF2K; elongation factor 2 kinase; elongation factor-2 kinase; euk; eukaroytic elongation factor 2 kinase; eukaryotic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; HSU93850; kinase eEF2K; SMEF2K | |
| Rabbit | |
| Antigen affinity chromatography | |
| RUO | |
| 13631, 25435, 29904 | |
| -20°C, Avoid Freeze/Thaw Cycles | |
| Liquid |
| Western Blot | |
| 0.75 mg/mL | |
| PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide | |
| O00418, O08796, P70531 | |
| EEF2K | |
| Phospho-eEF2K (Ser-500) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-500 in human eEF2K. This sequence is well conserved in rat and mouse eEF2K, and has low homology to other proteins. | |
| 100 μL | |
| Primary | |
| Human, Mouse, Rat | |
| Antibody | |
| IgG |
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