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  6. Invitrogen™ Phospho-Histone H2A.X (Ser139) Recombinant Mouse Monoclonal Antibody (CR55T33), Alexa Fluor™ Plus 555

Invitrogen™ Phospho-Histone H2A.X (Ser139) Recombinant Mouse Monoclonal Antibody (CR55T33), Alexa Fluor™ Plus 555

Product Code. p-200087426
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20 μL
50 μL
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20µg
50µg
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30245445 50 μL 50µg
30245418 20 μL 20µg
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Product Code. 30245445 Supplier Invitrogen™ Supplier No. 740078MP555

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Description

Mouse Recombinant Monoclonal Antibody

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Applications Tested: This 740078MP555 antibody (CR55T33) has been tested by western blot and Flowcytometry analysis of Jurkat cells treated with 50 μM etiposide for 8 hrs. This may be used for western blotting at 2 μg/mL and for western blotting. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser Filtration: 0.2 μm post-manufacturing filtered.

Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Phospho-Histone H2A.X (Ser139)
Applications Flow Cytometry, Western Blot
Classification Recombinant Monoclonal
Clone CR55T33
Concentration 1.0 mg/mL
Conjugate Alexa Fluor Plus 555
Formulation Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8
Gene H2AX
Gene Accession No. P16104
Gene Alias AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2A.X; H2A.X variant histone; H2A/X; h2afx; H2ax; H2AX histone; Hist5-2ax; histone 5 protein 2ax; histone H2A.x; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329
Gene Symbols H2AX
Host Species Mouse
Purification Method Protein A/G
Quantity 50 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3014
Target Species Human
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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