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Thermo Scientific™ Phusion Blood Direct PCR Master Mix

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

€ 119.00 - € 514.00

Specifications

GC-Rich PCR Performance High
PCR Method Direct PCR
Polymerase Phusion Blood II DNA Polymerase
Reaction Speed Fast
Includes Label or Dye Yes
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Products 2
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
15280114
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Thermo Scientific™
F175S
100 Reactions
€ 119.00
100 reactions
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15290114
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Thermo Scientific™
F175L
500 Reactions
€ 514.00
500 reactions
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Description

Description

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.

This accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Master Mix an ideal choice for demanding applications, such as cloning. Phusion Blood Direct PCR Master Mix also allows analysis of blood samples with different preservatives (e.g., heparin, EDTA, citrate), storage conditions, and from various species.

Highlights

  • Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
  • Available Dilution & Storage protocol allows several PCR reactions from one sample
  • Specially engineered Phire Hot Start II DNA polymerase for extremely short PCR protocol times
  • Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing
  • Cloning

Notes

  • Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
Specifications

Specifications

High
Phusion Blood II DNA Polymerase
Yes
Wet or Dry Ice
2X
Blunt
Blood
Direct PCR
Fast
Direct PCR Master Mix
Genotyping
Built-In Hot Start
SuperMix or Master Mix
DNA
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.