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Thermo Scientific™ Pierce™ Cypridina Luciferase Glow Assay Kit
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The Thermo Scientific™ Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.
Highlights:
Sensitive – highly sensitive detection of Cypridina luciferase activity
Secreted luciferase – allows real-time assays and kinetic studies without destroying cells
Stable – reagents provide increased signal stability compared to flash assays
Simple – does not require a luminometer with injectors
Compatible – assay reagents compatible with other Cypridina or Vargula luciferases
Automation-friendly – amenable to high throughput assays
Convenient – contains a universal cell lysis buffer and optimized glow assay reagent
Safe – allows one to perform non-radioactive assays
Includes:
Cell lysis buffer, reaction buffer and substrate
Requires:
Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers
Recommended for:
Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies
Spezifikation
Spezifikation
| Type | Assay Kit |
| Target | Luciferase, Cypridina Luciferase |
| Quantity | 100 Reaction Kit |
| Content And Storage | • Cypridina Glow Assay Buffer, 5 mL, store at 4°C • Vargulin (1X), 5 μL, store at -20°C • 2X Cell Lysis Buffer, 6 mL, store at room temperature Upon receipt store kit at -20°C or store individual components as indicated above. |
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Compatible Cells | Mammalian Cells |
| Detection Method | Bioluminescence |
| For Use With (Equipment) | Luminometer (Microplate) |
| Format | 384-well plate, 96-well plate |
| Label Type | Enzyme Labeled |
| Mehr anzeigen |
Sicherheit und Handhabung
- Pierce Cypridina Luciferase Glow Assay Kit
- Danger
- Acute toxicity Category 3
- Serious eye damage/eye irritation Category 1
- Flammable liquid Category 2
- Skin corrosion/irritation Category 1 A
- Specific target organ toxicity after single exposure Category 1
- H225-Highly flammable liquid and vapour.
- H301-Toxic if swallowed.
- H311-Toxic in contact with skin.
- H314-Causes severe skin burns and eye damage.
- H331-Toxic if inhaled.
- H370-Causes damage to organs.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P233-Keep container tightly closed.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P243-Take precautionary measures against static discharge.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P271-Use only outdoors or in a well-ventilated area.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P363-Wash contaminated clothing before reuse.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : methanol
For Research Use Only. Not for use in diagnostic procedures.
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