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Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Brand:  Thermo Scientific™ 32106

Additional Details : Weight : 0.70000kg

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Product Code. 10005943

  • € 255.00 / 500mL

In stock, ready to ship: 119
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Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard enhanced chemilumescent (ECL) substrates for detection of horseradish peroxidase (HRP) enzyme activity, yet costs much less. Because the luminol and peroxide reagent-formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Simply switch to Pierce ECL and save a bundle.


  • ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

  • About half the cost of other ECL Substrates – low overhead and a commitment to customer value enables Thermo Fisher Scientific to make this product available to you at a significantly lower price than other companies (these claims are based on the 2010 U.S. list prices)

  • No optimization required – switching to Pierce ECL substrate from other entry-level ECL substrates does not require optimization or protocol changes

  • A name you can can rely on – count on the strong quality assurance, technical support and reputation behind Thermo Scientific Pierce Protein Research Products


Detection Reagent 1 and 2



1 to 2 hr
Pierce ECL Western Blotting Substrate
Luminol/Enhancer, 250mL, Stable Peroxide Buffer, 250mL
Low picogram
50 mini-gel blots; 4000cm2 of membrane
2°: 1:1-15K (0.07-1µg/mL), 1°: 1:1K (0.2- 10µg/mL) 
Chemical Substrate
HRP (Horseradish Peroxidase) Substrate
Upon receipt store at 2-8°C. Product is shipped at ambient temperature.
Proprietary luminol and peroxide solutions
500 mL Kit
2°C to 8°C