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Thermo Scientific™ Pierce™ Magnetic RNA-Protein Pull-Down Kit
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Utilize a streamlined, robust method to enrich for protein-RNA interactions using end-labeled RNA as the bait with Thermo Scientific™ Pierce Magnetic RNA-Protein Pull-Down Kit.
Additional Information and Online Ordering
The Magnetic RNA-Protein Pull-Down Kit provides reagents to efficiently enrich RNA Binding Proteins (RBPs) using RNA end-labeled with desthiobiotin and streptavidin magnetic beads. The complete kit contains sufficient reagents for 20 RNA labeling reactions and 20 protein-RNA pull-down assays. This direct enrichment of the protein-RNA interaction provides an alternative to antibody capture of protein-RNA complexes or moieties incorporated into the nucleic acid. An added advantage to the kit is that it includes validated controls for both the labeling and pull-down assay. The kit is amenable to several downstream applications, including Western blotting and Mass Spectrometry (MS).
Highlights:
- Direct – capture ribonucleoprotein complexes directly with end-labeled RNA; does not use or require antibodies for pull-down
- Easy to use – no spin cups or centrifugation necessary for the enrichment of RBP; procedure streamlined for minimal hands-on time (less than 3 hours) after RNA labeling reaction
- Flexible – use in vitro transcribed RNA or synthetic RNA for labeling of various lengths and complexity; proteins successfully enriched using endogenous, over-expressed, and in vitro translated lysates
- Specific – low bead background; unrelated RNA or mutated RNA does not significantly enrich specified RBPs
- Economical – less expensive than purchasing commercially synthesized end-labeled RNA, magnetic beads, and reagents separately
- Complete – contains both labeling and enrichment modules with buffers necessary for assay; positive control RNA, negative control RNA, and RBP antibody included
Includes:
Complete kit contains the Pierce RNA 3'-End Desthiobiotinylation Kit, positive and negative RNA controls, nucleic-acid compatible streptavidin magnetic beads, and buffers for RBP enrichment and elution
Requires:
User-supplied RNA and lysate (experimental sample)
Recommended for:
- Mutational analysis
- Structure function analysis
- Identification of RNA:Protein interactions
- MS analysis of unknown RNA:protein binding pairs or complexes
Specifications
Specifications
| Capacity (Metric) | 1 mL |
| Kit Contents | 50 pmol of RNA per Reaction |
| Content And Storage | Sufficient For: 20 RNA-protein complex pull-down reactions Slurry: 10mg/mL, 1% solids • Pierce Nucleic Acid-Compatible Streptavidin Magnetic Beads, 1 mL (store at 4°C) • RNA Capture Buffer (1X), 10 mL (store at 4°C) • Tris (20 mM, pH 7.5), 5 mL (store at 4°C) • Protein-RNA Binding Buffer (10X), 1 mL (store at 4°C) • Wash Buffer (1X), 10 mL (store at 4°C) • Biotin Elution Buffer, 1.5 mL (store at 4°C) • HuR Monoclonal Antibody (Mouse), 50 μL (store at 4°C) • Positive RNA Control (AR RNA), 250 pmol (store at -20°C) • Negative RNA Control (polyA25 RNA), 250 pmol (store at -20°C) • Pierce RNA 3' End Desthiobiotinylation Kit (part number 20163; store at -20°C) |
| Product Line | Pierce™ |
| Quantity | 20 reactions kit |
| Type | RNA-Protein Pull Down Kit |
| Sufficient For | 20 Reactions |
| Format | Kit |
For Research Use Only. Not for use in diagnostic procedures.
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