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Thermo Scientific™ NBT Substrate Powder (nitro-blue tetrazolium chloride)

Product Code. 10046213 Shop All Thermo Scientific Products
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Quantity:
1 g
Unit Size:
1g
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Product Code. 10046213

Brand: Thermo Scientific™ 34035

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This item is not returnable. View return policy

Description

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

Thermo Scientific™ Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).

Highlights:

  • AP substrate – for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples

  • Chromogenic – no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph

  • Package options – choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor

1-Step™ NBT/BCIP

  • Ideal for immunoblotting applications
  • Minimal assay-to-assay variability

1-Step NBT/BCIP Plus Suppressor

  • Ideal for staining tissues assayed with calf intestinal alkaline phosphatase without the interference caused by endogenous phosphatases
  • Contains 1mM levamisole to inhibit endogenous phosphatase activity
TRUSTED_SUSTAINABILITY

Specifications

Description Pierce NBT Substrate
Product Type Substrate
Detection Method Colorimetric
Form Powder
Membrane Compatibility Nitrocellulose, PVDF
Quantity 1 g
Substrate Alkaline Phosphatase
Reactivity Alkaline Phosphatase
Content And Storage Upon receipt store product at 4°C protected from moisture. Product is shipped at ambient temperature.

Safety and Handling

compliance-icons
Product Identifier
  • NBT Substrate Powder (nitro-blue tetrazolium chloride)
 Signal Word
  • Warning
 Hazard Category
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

For Research Use Only. Not for use in diagnostic procedures.

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