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Description
Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.
Features of Protein-Free Blocking Buffer include:
- Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
- Applications—compatible with western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
- Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
- High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)
Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.
Tekniske data
Tekniske data
| Chemical Name or Material | Protein-Free (TBS) |
| Concentration | 1X |
| For Use With (Application) | ELISA |
| Physical Form | Liquid |
| Quantity | 100 mL |
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