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Thermo Scientific™ Pierce™ Recombinant Protein G

Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.

€ 508.00 - € 2580.00

Specifications

Product Line Pierce™
Form Powder
Protein Subtype Protein G
Target Antibodies
Content And Storage Upon receipt store at -20°C. Product is shipped at ambient temperature.
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Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10712725
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Thermo Scientific™
21193
5 mg
€ 508.00
5mg
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15285683
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Thermo Scientific™
77675
50 mg
€ 1018.00
50mg
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Estimated Shipment: 10-04-2024
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15295683
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Thermo Scientific™
77676
500 mg
€ 2580.00
500mg
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Estimated Shipment: 11-04-2024
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Description

Description

Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein G enable probing and detection of mouse and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

Protein G is a bacterial cell wall protein isolated from group G Streptococci. DNA sequencing of native Protein G identifies two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also effective at pH 7.0 to 7.2.

Highlights:

  • Contains two Fc-binding domains per protein
  • Better than Protein A for mouse (incl. IgG1), human (incl. IgG3) and rat, goat and cow antibodies
  • Poorer than Protein A for guinea pig, pig, dog and cat
  • Does not bind human IgM, IgD or IgA
Specifications

Specifications

Pierce™
Protein G
Upon receipt store at -20°C. Product is shipped at ambient temperature.
Powder
Antibodies
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.