missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ STAT3 Multispecies In-Cell ELISA Kit, Near Infrared

Measure total and phosphorylated signal transducers and activators of transcription 3 using antibodies detected by fluorescence or TMB substrate in whole-cell plate assays.

Brand:  Invitrogen™ 62214

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 11893845

  • € 562.00 / Each


Estimated Shipment: 12-07-2021
Add to basket

Description

Description

Thermo Scientific™ Pierce STAT3 In-Cell ELISA Kits are colorimetric and fluorescent microplate assays optimized to measure total and phosphorylated signal transducers and activators of transcription 3 (phospho-STAT3) in whole cells.

STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.
Specifications

Specifications

P40763
ELISA Kit
Pierce STAT3 Near Infrared In-Cell ELISA Kit
Blocking Buffer, 20X Tris Buffered Saline, Surfact-Amps 20, Surfact-Amps X-100, DyLight™ 680 Goat Anti-Mouse IgG (H+L), DyLight 800 Goat Anti-Rabbit IgG (H+L), Thin Plate Seal Assembly, Components included only in the target-specific kits:Antibody #1 and Antibody #2, Anti-STAT3 Antibody
Supernatant, 10,000 cells
96 microplate wells of overnight-cultured cells
24 hr.
96 Tests
Supernatant
Multi-component kit with fluorescent conjugates
DyLight™ 680, DyLight™ 800
Unconjugated
6774
RUO
2°C to 8°C
1 hr. 20 min.
Human
Human
ADMIO,ADMIO1,APRF,HIES
Fluorescent Microplate Reader
Videos
SDS
Documents
Certificates