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Description
Obtain fast, simple, and highly reproducible chymotrypsin protein digestion for peptide characterization and quantitation in biopharmaceutical research.
Obtain high-quality analytical results from protein digests using Thermo Scientific™ SMART Digest™ Chymotrypsin Kits. A significant advance in sample preparation for biopharmaceutical protein research, these kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. SMART Digest Kits are a significant improvement over in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.
Characterize and quantitate proteins faster, easier, and quicker than ever before using SMART Digest kits. This version of the kit uses chymotrypsin, which cleaves peptide bonds formed by aromatic residues such as tyrosine, phenylalanine, and tryptophan.
Easy and Simple- Significantly increased speed of sample processing
- Simple process
- Easily automate entire protocol for high-throughput processing
- Increased reproducibility over existing protocols
- Higher sensitivity
- Higher data confidence
Typically it takes less than 60 minutes (dependent on sample complexity) to achieve full digestion, whereas an in-solution digestion can take up to 24 hours.
Cleaner SamplesReduction in the number of chemicals used and the immobilized chymotrypsin design helps mitagate against chemically induced post translational modifications (PTMs) and autolysis. The results are easier to interpret.
Couple with SOLAμ SPE plates for desaltingWhen used with the SMART Digest for desalting, Thermo Scientific™ SOLAμ™ SPE solid phase extraction plates provide a fast, easy-to-use, robust, and easily automatable sample preparation solution for analysis of biomolecules.
Spécification
Spécification
| Description | Low pH, Magnetic Beads, Bulk option for MAM workflows |
| Capture | None |
| Cleavage | Peptide bonds of aromatic residues (e.g., tyrosine, phenylalanine, tryptophan) |
| Enzyme | Chymotrypsin |
| Format | SMART Digest Chymotrypsin, Bulk Magnetic |
| For Use With (Application) | Peptide Mapping for MAM workflows |
| Product Line | SMART Digest |
| Type | Protein Digestion |
| Includes | SMART Digest Resin Bulk Magnetic, SMARRT Digest Buffer (low pH) |
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