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Eurofins DiscoverX PKC θ Protein, Active

Product Code. 17330184
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Quantity:
10 μg
250 μg
Unit Size:
10µg
250µg
This item is not returnable. View return policy

Product Code. 17330184

Brand: Eurofins DiscoverX 14444M

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This item is not returnable. View return policy

Description

Active, recombinant full-length human PKC theta with a C-terminal 6His-tag, for use in Kinase Assays.

Protein kinase C, theta (PKCtheta, PRKCQ, PRKCT) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphophate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.

GenBank L07032

Specifications

Formulation mg/mL of enzyme in 50 mM Hepes/NaOH pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 5% glycerol, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Molecular Weight (g/mol) 82.8 kDa
Quantity 250 μg
Species Human
Recombinant Recombinant
Protein Tag C-terminal 6His
Expression System Recombinant enzyme expressed in Sf21 insect cells
Protein Form Full-length
Purity or Quality Grade ≥55%
Protein PKC Theta, active
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