Platelet GPVI Mouse anti-Human, BUV395, Clone: HY101 (also known as HY 101; HY-101), BD Optibuild™

Description

The HY101 monoclonal antibody specifically binds to a collagen receptor, Platelet Glycoprotein VI (GPVI or GP6). GPVI is a 58-kD platelet membrane glycoprotein that belongs to the immunoglobulin superfamily. Upon blood vessel wall injury and consequent endothelial cell damage, platelets collect upon the exposed collagen fibers present in the subendothelial matrix. The platelet and collagen interaction leads to activated platelet aggregation and thrombus formation. Although normally serving a protective function in clot formation that limits blood loss, the platelet GPVI/collagen interaction may also play a role in myocardial infarction and stroke which are associated with the rupture of atherosclerotic plaques.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.