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Merck Millipore Proteinase K
From Tritirachium album
Brand: Merck Millipore 1245680500
Additional Details : CAS : 39450-01-6 Weight : 0.00050kg
Description
- Chromatographically purified lyophilized 30 m Anson-U/mg
- For biochemistry and molecular biology EC 3.4.21.14
- Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
- Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
- Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
- Determination of enzyme localization on membranes
- Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
- Digestion of proteins from brain tissue samples for prions in Transmissible
- Spongiform Encephalopathies (TSE) research.
- Proteinase K is a stable and highly reactive serine protease.
- Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224).
- It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature.
- In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
Specifications
| 6.2 to 6.8 (20°C, 10 g/L in H2O) | |
| C29H27N2O12P | |
| CC(O)C1C(=O)N2C(C(=O)OCC3=CC=C(C=C3)[N+]([O-])=O)=C(OC3=CC=CC=C3)C(C)(OC3=CC=CC=C3)C12OP(O)(O)=O | |
| 626.51 | |
| Endopeptidase K, Proteinase K from Tritirachium album, Tritirachium alkaline proteinase |
| 39450-01-6 | |
| OUPAKHMWUJJXOM-UHFFFAOYNA-N | |
| {[6-(1-hydroxyethyl)-4-methyl-2-{[(4-nitrophenyl)methoxy]carbonyl}-7-oxo-3,4-diphenoxy-1-azabicyclo[3.2.0]hept-2-en-5-yl]oxy}phosphonic acid | |
| 500 mg |