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Invitrogen™ PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit

The PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane.

€ 148.00 - € 884.00

Specifications

Content And Storage • Resuspension buffer
• Lysis indicator
• RNase A
• Lysis buffer
• Precipitation buffer
• Endotoxin removal buffer
• Wash buffer
• Elution buffer
• TE buffer
• Expi Maxi filtration column
• Expi Maxi DNA binding column
• 50 mL conical tubes

Endotoxin-free components: elution buffer, water, plasmid storage tubes

Format Spin Column
Isolation Technology Anion Exchange Resin
Sample Type Bacteria
Scale Maxi
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Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15756003
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Invitrogen™
A33073
4 Preps
€ 148.00
Pack of 4
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15722362
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Invitrogen™
A31217
10 Preps
€ 356.00
Pack of 10
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15768227
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Invitrogen™
A31231
25 Preps
€ 884.00
25 preps
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Description

Description

The PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane. In as little as 90 minutes, purify up to 1.5 mg of high-quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines or genome engineering.

Advantages of the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit include:
• Simple and fast protocol—purification typically takes 90 minutes
• High yield—isolate up to 1.5 mg of high quality plasmid DNA
• Endotoxin-free plasmid—less than 0.1 EU/μg, ideal for sensitive applications
• High purity—ultralow levels of protein, RNA, and genomic DNA contamination
• Compatibility—suitable for use with ExpiCHO and Expi293 expression systems
• Endotoxin-free components— elution buffer, water, and plasmid storage tubes that are free of endotoxin

Simple and fast protocol
Unlike other anion-exchange-based endotoxin-free plasmid purification systems, the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit utilizes centrifugation- or vacuum-assisted filtration for lysate clarification and subsequent plasmid DNA purification. There is no waiting for gravity-flow columns and the entire purification typically takes 90 minutes (including the final precipitation step).

Endotoxin-free plasmid
Purify endotoxin-free (less than 0.1 EU/μg) plasmid that is ideal for sensitive applications such as transfection of primary cells, genome engineering, and research on gene therapy or plasmid vaccines in vivo.

Compatible with ExpiCHO and Expi293 expression systems
Plasmid purified using the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit is fully compatible with the Gibco ExpiCHO and Expi293 transient mammalian expression systems.

Superior quality with endotoxin-free components
Rest assured that the kit is of high quality and has additional endotoxin-free elution buffer, water, and plasmid storage tubes for added peace of mind.

Specifications

Specifications

• Resuspension buffer
• Lysis indicator
• RNase A
• Lysis buffer
• Precipitation buffer
• Endotoxin removal buffer
• Wash buffer
• Elution buffer
• TE buffer
• Expi Maxi filtration column
• Expi Maxi DNA binding column
• 50 mL conical tubes

Endotoxin-free components: elution buffer, water, plasmid storage tubes

Anion Exchange Resin
Maxi
Cloning, PCR
> 1 mg (Large-Scale) Plasmid DNA, > 200 μg (Large-Scale) Plasmid DNA
Plasmid Purification Kit
Plasmid DNA
Spin Column
Bacteria
Mammalian
Manual Protocols
PureLink™
Room Temperature
90 min.
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.