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Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit
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No. of Reactions:
25 Preps
50 Preps
Unit Size:
Pack of 25
Pack of 50
Description
The simple precipitation-free protocol yields up to 0.4 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.
- Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
- Low endotoxin plasmid—the resulting plasmid contains less than 1 EU/μg endotoxins
- High yields—isolate up to 0.4 mg of high-quality plasmid DNA
- Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization
Specifications
Specifications
| Content And Storage | • Resuspension buffer, store at 4°C upon reciept • Lysis buffer, store at room temperature • Precipitation buffer, store at room temperature • Binding buffer, store at room temperature • Wash buffer 1, store at room temperature • Wash buffer 2 (concentrate), store at room temperature • Elution buffer, store at room temperature • Column assemblies, store at room temperature • Syringe filters, store at room temperature • Syringe plungers, store at room temperature • Collection tubes, store at room temperature |
| Format | Kit |
| Isolation Technology | Silica Column |
| Scale | Midi |
| For Use With (Application) | Cloning, PCR |
| No. of Reactions | 50 Preps |
| Prep Scale | Midi scale input culture of 25-100 mL to obtain yields of up to 200 μg |
| Product Line | PureLink™ |
| Product Type | Plasmid Purification Kit |
| Quantity | 50 Preps |
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Safety and Handling
Product Identifier
- PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit
- Danger
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H225-Highly flammable liquid and vapour.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H336-May cause drowsiness or dizziness.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P271-Use only outdoors or in a well-ventilated area.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
- P403+P233-Store in a well-ventilated place. Keep container tightly closed.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Sodium hydroxide, Isopropanol
For Research Use Only. Not for use in diagnostic procedures.
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