Invitrogen™ RAD18 Polyclonal Antibody

Beskrivning

Immunogen sequence: LESPAKSPAS SSSKNLAVKV YTPVASRQSL KQGSRLMDNF LIREMSGSTS ELLIKENKSK FSPQKEASPA AKTKETRSVE EIAPDPSEAK RPEPPSTSTL KQVTKVDCP Highest antigen sequence identity to the following orthologs: Mouse - 52%, Rat - 56%.

Human Rad18 (hRad18) is homologue of the S. pombe Rad18 (3,4). In yeast, Rad6 and Rad18 play a crucial role in post-replication repair functions in gap-filling of a daughter strand on replication of damaged DNA (2). Furthermore, Rad6-Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. In vivo, Rad18 protein forms a tight complex with Rad6 through a conserved ring-finger motif (3,4). It has been suggested that Rad18-Rad6 complex binds to the damaged sites by the single-stranded DNA binding property of Rad 18. This is followed by degradation of the stalled components of DNA replication by ubiquitin-conjugating enzyme activity of Rad6 (1). The human Rad18 maps on chromosome 3p24-25, the region which is often deleted in lung, breast, ovary, and testis cancers. The human RAD18 gene codes for a protein of 495 amino acids with a predicted molecular weight of 56 kDa. The human Rad18 is ubiquitously expressed in various human tissues.