Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 450, eBioscience™, Invitrogen™

Description

Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin. Applications Reported: Rat IgG1 Isotype Control eFluor® 450 has been reported for use in flow cytometric analysis. Applications Tested: eFluor® 450 Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as experimental antibody. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.

Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics. These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants. Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens. It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens. Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases. With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.