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  5. Invitrogen™ Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 645, eBioscience™

Invitrogen™ Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 645, eBioscience™

Product Code. 15875683
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Quantity:
100 μg
Unit Size:
100µg
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Product Code. 15875683 Supplier Invitrogen™ Supplier No. 64432182

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Description

Rat Monoclonal Isotype Control

The monoclonal rat IgG2a is useful as an isotype control immunoglobulin. Applications Reported: This eBR2a antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBR2a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes. Use the isotype control at the same concentration as the experimental antibody. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determi...

Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Rat IgG2a kappa
Applications Control, Flow Cytometry
Classification Monoclonal
Clone eBR2a
Concentration 0.2 mg/mL
Conjugate Super Bright 645
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Host Species Rat
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Isotype Control (Primary)
Target Species Not Applicable
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Isotype Control
Form Liquid
Isotype IgG2a κ
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