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  5. Invitrogen™ Rat IL-10 ELISA Kit

Invitrogen™ Rat IL-10 ELISA Kit

Product Code. 15562067
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Quantity:
2 x 96 Tests
5 x 96 Tests
Unit Size:
Pack of 2
Pack of 5
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Product Code. 15562067

Brand: Invitrogen™ BMS629TWO

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Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to rat IL-10
Biotin-Conjugate anti-rat IL-10 monoclonal antibody
Streptavidin-HRP
Rat IL-10 Standard lyophilized, 2ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

ELISA

The Rat Interleukin-10 (Rt IL-10) ELISA quantitates Rt IL-10 in rat serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt IL-10.

Principle of the method The Rat IL-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P29456
Assay Range 15.6-1000 pg/mL
Assay Sensitivity 1.5 pg/mL
Content And Storage Aluminum pouch(es) with a Microplate coated with monoclonal antibody to rat IL-10
Biotin-Conjugate anti-rat IL-10 monoclonal antibody
Streptavidin-HRP
Rat IL-10 Standard lyophilized, 2 ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Serum, Supernatant
Target Species Rat
For Use With (Application) ELISA
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIFCSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF; IL10X
Gene ID (Entrez) 25325
Gene Symbol IL10
Interassay CV 10%
Intraassay CV 5%
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Protein Aliases IL-10, Cytokine synthesis inhibitory factor, CSIF
Quantity 2 x 96 Tests
Regulatory Status RUO
Sample Volume Serum 50 μL, Supernatant 50 μL
Storage Requirements 2-8°C
Synonym TGIF, MCSF III, CSIF, B TCGF
Target IL-10
Test Time 1 hr 20 min
Total Assay Time 3 hr 10 min
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