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R&DSystems™ Recombinant Human Aminopeptidase N/CD13 His Avi Protein, CF
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10 μg
Description
Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >5000 pmol/min/μg, as measured under the described conditions. Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human Aminopeptidase N/CD13 His-tag Avi-tag (Catalog # AVI3815) binds Human Aminopeptidase N/CD13 Antibody (Catalog # AF3815 ) with an ED50 of 1.80-18.0 ng/mL.
Specifications
Specifications
| For Use With (Application) | Enzyme Activity |
| Formulation | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
| Gene ID (Entrez) | 290 |
| Molecular Weight (g/mol) | M.W.-observed: 116-130 kDa, under reducing conditions., M.W.-theroretical: 105 kD |
| Quantity | 10 μg |
| Source | Chinese Hamster Ovary cell line, CHO-derived human Aminopeptidase N/CD13 protein Human APN (Lys69-Lys967) (N-terminus) Accession # AAA51719.1 6-His tag Avi-tag (C-terminus) |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening. |
| Endotoxin Concentration | <0.10 EU per 1 μg of the protein by the LAL method. |
| Gene Alias | alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Aminopeptidase M, aminopeptidase N, ANPEP, AP-M, APN, AP-N, CD13, CD13 antigen, CD13APN, EC 3.4.11, EC 3.4.11.2, gp150, LAP1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, P150, PEPN, PEPNhAPN |
| Conjugate | Biotin |
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