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R&D Systems™ Recombinant Human Chitinase 3-like 1/YKL-40 Protein
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Quantity:
50 μg
Unit Size:
50µg
Description
Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. When 5 x 104 cells per well are added to recombinant human CHI3-L1 coated plate, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.6-2.4 μg/mL.
Specifications
Specifications
| Accession Number | P36222 |
| For Use With (Application) | Bioactivity |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Gene ID (Entrez) | 1116 |
| Molecular Weight (g/mol) | M.W. (Observed): 38-41 kDa, reducing conditions; M.W. (theoretical): 41.3 kDa (monomer) |
| Name | Chitinase 3-like 1 |
| Quantity | 50 μg |
| Source | Mouse myeloma cell line, NS0-derived human Chitinase 3-like 1 protein Tyr22-Thr383, with a C-terminal 6-His tag |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. |
| Endotoxin Concentration | <0.10 EU / 1 μg of the protein by the LAL method. |
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