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R&D Systems™ Recombinant Human PDGF-AA, Animal-Free Protein

Product Code. 18768486
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Quantity:
10 μg
50 μg
Unit Size:
10µg
50µg
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Product Code. 18768486

Brand: R&D Systems™ AFL221010

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This item is not returnable. View return policy

Description

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. ED50 for this effect is 50 to 200 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number P04085.1
For Use With (Application) Bioactivity
Gene ID (Entrez) 5154
Molecular Weight (g/mol) M.W. theroretical: 14 kDa (monomer)
Quantity 10 μg
Source E. coli-derived human PDGF-AA protein Ser87-Thr211, with and without an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, ≤ -20°C under sterile conditions after reconstitution.
Gene Alias PDGF1, PDGF-A, PDGFAA
Gene Symbol PDGFA
Conjugate Unconjugated
Species Human
Reconstitution Reconstitute at 0.2 mg/mL in sterile 4 mM HCl.
Buffer Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Endotoxin Level <0.01 EU per 1 μg of the protein by the LAL method.
Purity or Quality Grade >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
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