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Description
LIGHT/TNFSF14 protein is fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using human HT-29 cells is less than 2 μg/mL, corresponding to a specific activity of > 500 IU/mg in the presence of murine anti-polyHistidine monoclonal antibody and rHuIFN-g.
Specifications
Specifications
| For Use With (Application) | Western Blot, Bioactivity |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Gene ID (Entrez) | 8740 |
| Molecular Weight (g/mol) | MolecularWeight-theroretical: 18.4 kDa |
| Quantity | 500 μg |
| Storage Requirements | Store at -20°C to -70°C as supplied. After reconstitution, store at 2 to 8 C for 1 month and (at -20°C to -70°C for long term storage. Avoid repeated freeze/thaw cycles.) |
| Endotoxin Concentration | Less than 1 EU/μg of LIGHT/TNFSF14 as determined by LAL method. |
| Gene Alias | CD258, CD258 antigen, delta transmembrane LIGHT, Herpes virus entry mediator ligand, Herpesvirus entry mediator ligand, HVEM-L, HVEMLherpesvirus entry mediator-ligand, ligand for herpesvirus entry mediator, LIGHTherpesvirus entry mediator A, LTg, TR2, tumor necrosis factor (ligand) superfamily, member 14, tumor necrosis factor ligand superfamily member 14, tumor necrosis factor receptor-like 2, tumor necrosis factor superfamily member LIGHT |
| Gene Symbol | TNFSF14 |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in 100 mM HAC to a concentration of 0.1-1.0 mg/mL Apportion stock solutions into working aliquots and store (at <-20°C.) |
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