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  4. enQuireBio™ Recombinant other LLO Protein

enQuireBio™ Recombinant other LLO Protein

Product Code. 15985118
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Quantity:
10 μg
100 μg
50 μg
Unit Size:
100µg
10µg
50µg
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Product Code. 15985118

Brand: enQuireBio™ QP1077310ug

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This item is not returnable. View return policy

Description

A cDNA sequence encoding the LLO was constructed and used to recombinantly synthesize the protein.

Specifications

Name LLO Protein
Quantity 10 μg
Regulatory Status Research Use Only
Endotoxin Concentration < 1.0 EU per ug protein as determined by the LAL method.
Gene Symbol hlyA
Biological Activity Hemolytic activity for Recombinant Listeriolysin O was 1.18 x 105 HU/mg of protein where HU means hemolytic activity unit that is the amount of toxin needed to release half the hemoglobin (50% lysis) of the erythrocytes as determined by hemolysin assay. 2mM DTT is used to reactivate the toxin.
Product Type Recombinant Protein
Cross Reactivity Other
Species E. coli
Protein Tag Untagged
Sequence MKKIMLVFIT LILISLPIAQ QTEAKDASAF HKEDLISSMA PPTSPPASPK TPIEKKHADE IDKYIQGLDY NKNNVLVYHG DAVTNVPPRK GYKDGNEYIV VEKKKKSINQ NNADIQVVNA ISSLTYPGAL VKANSELVEN QPDVLPVKRD SLTLSIDLPG MTNQDNKIVV KNATKSNVNN AVNTLVERWN EKYAQAYPNV SAKIDYDDEM AYSESQLIAK FGTAFKAVNN SLNVNFGAIS EGKMQEEVIS FKQIYYNVNV NEPTRPSRFF GKAVTKEQLQ ALGVNAENPP AYISSVAYGR QVYLKLSTNS HSTKVKAAFD AAVSGKSVSG DVELTNIIKN SSFKAVIYGG SAKDEVQIID GNLGDLRDIL KKGATFNRET PGVPIAYTTN FLKDNELAVI KNNSEYIETT SKAYTDGKIN IDHSGGYVAQ FNISWDEINY DPEGNEIVQH KNWSENNKSK LAHFTSSIYL PGNARNINVY AKECTGLAWE WWRTVIDDRN LPLVKNRNIS IWGTTLYPKY SNSVDNPIE
Buffer The protein contains 50mM NaH2PO4, 1mM EDTA, 2.7mM KCl, 1mM DTT, 5% (v/v) glycerol and 0.5M NaCl.
Purity or Quality Grade Greater than 90.0% as determined by SDS-PAGE.
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