ROR gamma (t), APC, clone: AFKJS-9, eBioscience™
Rat Monoclonal Antibody
Merk: Affymetrix eBioscience 17-6988-82
Overige gegevens : Gewicht : 0.25000kg
BeschrijvingDescription: The AFKJS-9 monoclonal antibody reacts with the mouse, human and rhesus monkey RORgamma(t) protein. RORgamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes RORalpha and RORbeta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. RORgamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, RORgammat, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ double-positive thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs. The RORgammat isoform differs from RORgamma by three unique amino acids at its amino terminus. Therefore, the AFKJS-9 antibody will react with both the RORgamma and RORgammat isoforms.Applications Reported: This AFKJS-9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This AFKJS-9 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This antibody can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|ROR gamma (t)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|MGC129539, NR1F3, RORG, RZR-GAMMA, RZRG, TOR|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.