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Gibco™ RPMI 1640 Medium

Originally developed to culture human leukemic cells in suspension and as a monolayer

Brand:  Gibco™ 21875091

Additional Details : Weight : 0.50000kg

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Product Code. 12004997

  • € 108.00 / Pack of 10


In stock, ready to ship: 302
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Description

Description

  • Originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
  • Unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g ⁄ L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
  • For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
  • Manufactured at a cGMP-compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical RPMI 1640 product made in our Grand Island facility (11875-119). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
  • Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications.
  • Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
  • RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Specifications

Specifications

1 X
Animal Origin-Free
No HEPES
260 - 310 mOsm/kg
Sodium Bicarbonate
10 x 500 mL
Liquid
Phenol Red
Sustainable packaging
7 to 7.4
No Sodium Pyruvate
RPMI 1640
CO2
7.0 - 7.4
Standard Serum Supplementation
Serum
Gibco™
L-Glutamine
12 Months
Mammalian Cell
Mammalian Cell Culture
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