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R&D Systems™ rS. equi subsp. zooepidemicus IgG endopeptidase IdeZ, CF

Product Code. 30226364
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:
50 μg
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Product Code. 30226364

Brand: R&D Systems™ 11572IZ050

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This item is not returnable. View return policy

Description

Recombinant Streptococcus His-tag

Measured by its ability to cleave Human IgG. The DC50 is <25 ng, as measured under the described conditions. The DC50 is defined as the amount of enzyme required to cleave 50% of 1 μg human IgG in 30 minutes at 37 °C. Use of Recombinant Streptococcus equi subsp. zooepidemicus IdeZ in the cleavage of other IgGs may required alternative conditions for optimal performance.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number WP_014622780.1
For Use With (Application) Enzyme Activity
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Molecular Weight (g/mol) M.W.-observed: 35-39 kDa, under reducing conditions, M.W.-theroretical: 36 kDa
Quantity 50 μg
Source E. coli-derived IdeZ protein Asp35-Ser349, with an N-terminal Met and C-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.
Endotoxin Concentration <0.10 EU per 1 μg of the protein by the LAL method.
Conjugate Unconjugated
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
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