Invitrogen™ Ryanodine Receptor Monoclonal Antibody (C3-33)

Description

MA3-916 detects ryanodine receptor 2 (RyR2) and shows cross-reactivity with RyR1 in amphibian, canine, chicken, fish, guinea pig and rat tissues. MA3-916 has been successfully used in Western blot, immunohistochemical, immunofluorescence, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 565 kDa protein representing the ryanodine receptor. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in rat cardiac tissue with MA3-916 results in intense staining of the myofiber, which is consistent with sarcoplasmic reticulum localization.

The ryanodine receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the 'foot' region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).