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Invitrogen™ SF1 Polyclonal Antibody
Rabbit Polyclonal Antibody
Brand: Invitrogen™ PA528054
This item is not returnable.
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Description
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (94%), Xenopus laevis (96%), Rabbit (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Splicing factor 1 is a nuclear pre-mRNA splicing factor. The protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
Specifications
| SF1 | |
| Polyclonal | |
| Unconjugated | |
| SF1 | |
| BBP; D11S636; M ZFM1; Mammalian branch point-binding protein; MBBP; MZFM; SF; SF1; Splicing factor 1; transcription factor ZFM1; WBP4; wu:fc09f06; ZCCHC25; ZFM1; Zfp162; Zinc finger gene in MEN1 locus; zinc finger protein 162; ZNF162 | |
| Rabbit | |
| Antigen affinity chromatography | |
| RUO | |
| 7536 | |
| Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
| Liquid |
| Immunoprecipitation, Western Blot, Immunocytochemistry | |
| 0.77 mg/mL | |
| 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7 | |
| Q15637 | |
| SF1 | |
| Recombinant protein encompassing a sequence within the center region of human splicing factor 1. The exact sequence is proprietary. | |
| 100 μL | |
| Primary | |
| Human | |
| Antibody | |
| IgG |
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