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Invitrogen™ SMCC Monoclonal Antibody (6-F6)

Description
Application notes: Indirect ELISA.
Succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC) is a non-cleavable and membrane permeable crosslinker. It contains an amine-reactive N -hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of SMCC and Sulfo-SMCC and are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm.
Specifications
Specifications
| Antigen | SMCC |
| Applications | ELISA |
| Classification | Monoclonal |
| Clone | 6-F6 |
| Concentration | 2 mg/mL |
| Conjugate | Unconjugated |
| Formulation | PBS with 0.2% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4 |
| Gene Alias | Succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate |
| Host Species | Mouse |
| Immunogen | Recombinant protein fused with SMCC. |
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