missing translation for 'onlineSavingsMsg'
Learn More
Learn More
IBA Lifesciences Strep-Tactin™ TACS Agarose
50% suspension for traceless affinity cell and exosome isolation
€ 132.00 - € 935.00
Specifications
| Binding Capacity | Biotin Binding Capacity > 40 nmol/ml resin |
|---|---|
| Formulation | 50% suspension in buffer, pH 8: 100 mM Tris-HCl pH 8.0, 1 mM EDTA, 150 mM NaCl, 0.01% sodium azide |
| Support Type | Crosslinked agarose |
| For Use With (Application) | For Packing of Gravity Flow Columns for Affinity Chromatographic Cell or Exosome Isolation |
| Product Code | Brand | Capacity (Metric) | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Capacity (Metric) | Price | Quantity & Availability | |||||
|
17247750
|
IBA Lifesciences
6-6350-002 |
4 mL |
€ 132.00
4mL |
Estimated Shipment: 13-05-2024
Log in to see stock. |
|||||
|
17257750
|
IBA Lifesciences
6-6350-010 |
20 mL |
€ 411.00
20mL |
Estimated Shipment: 13-05-2024
Log in to see stock. |
|||||
|
17267750
|
IBA Lifesciences
6-6350-025 |
50 mL |
€ 935.00
50mL |
Estimated Shipment: 13-05-2024
Log in to see stock. |
|||||
Description
- Strep-Tactin™ TACS Agarose contains agarose macrobeads coated with the streptavidin variant Strep-Tactin™.
- A special crosslinking permits the use for cell isolation and depletion approaches.
- It was developed for column-based Traceless Affinity Cell Selection (Fab-TACS™/Nano-TACS™).
- In this approach, Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin™ on the agarose matrix.
- After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin™ Agarose by biotin addition.
- In this way, the eluted cells are ready for further downstream applications.
- The same principle is applicable for exosome isolation (Fab-TACS™ for exosomes)
- Alternatively to Fab-Streps/Nano-Streps, it is possible to bind Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding) to this resin.
- This makes Strep-Tactin™ TACS Agarose also suitable for negative cell selection or depletion approaches.
- The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography.
- To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.
Specifications
| Biotin Binding Capacity > 40 nmol/ml resin | |
| 50% suspension in buffer, pH 8: 100 mM Tris-HCl pH 8.0, 1 mM EDTA, 150 mM NaCl, 0.01% sodium azide | |
| Crosslinked agarose | |
| For Packing of Gravity Flow Columns for Affinity Chromatographic Cell or Exosome Isolation |
For Research Use Only.