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Description
- 10x Buffer R is a 10x concentrated Strep-Tactin™ regeneration buffer containing HABA. HABA (2-[4-hydroxy-benzeneazo]benzoic acid) displaces desthiobiotin after the elution of Strep-tag™II or Twin-Strep-tag™ fusion proteins from the modified biotin-binding pocket of Strep-Tactin™.
- The yellow buffer turns red upon binding of HABA to Strep-Tactin™, clearly indicating the regeneration process and activity status of the column.
- Please note that 10x Buffer R is not applicable for regeneration of Strep-Tactin™XT resins, but it can be used to monitor the activity status after the regeneration.
Specifications
Specifications
| Chemical Name or Material | Regeneration Buffer |
| Color | Yellow |
| Concentration | 10X |
| For Use With (Application) | For The Regeneration of Immobilized Strep-Tactin |
| Quantity | 100 mL |
Safety and Handling
Product Identifier
- Buffer R (10x), Strep-Tactin Regeneration Buffer with HABA
- Warning
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Acute toxicity Category 4
- Specific target organ toxicity Category 3
- Specific target organ toxicity after repeated exposure Category 2
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
- H332-Harmful if inhaled.
- H372-Causes damage to organs.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap and water.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
For Research Use Only.
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