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  5. Thermo Scientific™ SuperSignal™ West Pico Chemiluminescent Substrate

Thermo Scientific™ SuperSignal™ West Pico Chemiluminescent Substrate

Product Code. 10445685 Shop All Thermo Scientific Products
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Quantity:
100 mL Kit
50 mL Kit
Sufficient For:
12 mini-gel blots/1000 cm2 membrane
6 mini-gel blots/500 cm2 membrane
Unit Size:
100mL
50mL
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Product Code. Quantity Sufficient For unitSize
10445685 50 mL Kit 6 mini-gel blots/500 cm2 membrane 50mL
10177533 100 mL Kit 12 mini-gel blots/1000 cm2 membrane 100mL
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Product Code. 10445685 Supplier Thermo Scientific™ Supplier No. 34079

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Description

Enhanced chemiluminescent (ECL) HRP substrate that provides low-picogram-level detection by Western blot analysis. These products will be discontinued on September 30th, 2017. Please switch to the longer-lasting, more-sensitive SuperSignal West Pico PLUS.

Thermo Scientific™ SuperSignal West Pico Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) substrate for horseradish peroxidase (HRP) enzyme that provides low picogram detection of proteins in Western blot applications.

SuperSignal West Pico Substrate emits light for longer and chemiluminescent signal at more than twice the intensity of other entry-level luminol-based horseradish peroxidase detection systems while also maintaining sensitivity and low background. In addition, the greater signal intensity is achieved using less primary and secondary antibody than typical ECL substrates. All of this for about 30% less cost than comparable products.

Highlights:

  • ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

  • Economy – costs less per milliliter than other similarly sensitive chemiluminescent substrates

  • Picogram sensitivity – highly sensitive for the rapid development of a wide range of protein levels

  • Stable light output – long-duration signal makes it possible to make multiple exposures to film

  • High intensity – signal is twice as strong as output from other luminol-based systems

  • Excellent stability – 24-hour working solution stability; 1-year kit stability at room temperature

  • Saves antibody – requires much less (more dilute) primary and secondary antibodies per blot

TRUSTED_SUSTAINABILITY

Specifications

Description SuperSignal West Pico Chemiluminescent Substrate
Detection Method Chemiluminescence
Quantity 50 mL Kit
Conjugate HRP
Sufficient For 6 mini-gel blots/500 cm2 membrane
Kit Contents Luminol/Enhancer, 25mL, Stable Peroxide Buffer, 25mL
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