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Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Product Code. 15513766 Shop All Thermo Scientific Products
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15513766 200mL
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Product Code. 15513766 Supplier Thermo Scientific™ Supplier No. 34577

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Description

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Thermo Scientific™ SuperSignal™ West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions making it an ideal choice for most western blotting applications.

Features of SuperSignal West Pico PLUS Chemiluminescent Substrate:
  • Picogram to femtogram sensitivity—detect low-picogram to high-femtogram amounts of target protein on nitrocellulose or PVDF membrane
  • High signal stability—incubated blots provide stable signal duration over the critical 4 hour time period with up to 24 hours of light output under optimal conditions
  • Stable reagent—8-hour working solution stability; 1-year kit stability at room temperature
  • Economical—optimized for dilute antibody concentrations:
    • 0.2 to 1.0μg/mL primary antibody (1:1000 to 1:5000 dilution from 1mg/mL stock)
    • 10 to 50ng/mL secondary antibody (1:20,000 to 1:100,000 dilution from 1mg/mL stock)
  • Exceptional robustness—provides high performance outside of the recommended antibody dilution ranges, including the most common 1:5K to 1:10K secondary antibody dilutions of a 1mg/mL stock solution

For Research Use Only. Not for use in diagnostic procedures.
TRUSTED_SUSTAINABILITY

Specifications

Product Type Substrate
Detection Method Chemiluminescence
Form Liquid
For Use With (Application) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line SuperSignal™
Quantity 200 mL
Conjugate HRP
Substrate Horseradish Peroxidase
Recommended Antibody Concentrations 2°: 1:20-100K (10-50ng/mL), 1°: 1:1K (0.2- 1μg/mL)
Sensitivity Picogram to high femtogram-level
Signal Duration 6 to 24 hr.
Sufficient For 25 Mini-Gel Blots, 2000 sq. cm of Membrane
Kit Contents SuperSignal West Pico PLUS Luminol/Enhancer, 100 mL, SuperSignal West Pico PLUS Stable Peroxide, 100 mL
Content And Storage Sufficient for 25 mini-gel blots; 2000 cm2 of membrane
• SuperSignal West Pico PLUS Luminol/Enhancer, 100 mL
• SuperSignal West Pico PLUS Stable Peroxide, 100 mL

Store at room temperature.
Shipping Condition Room Temperature
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For Research Use Only. Not for use in diagnostic procedures.

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