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Thermo Scientific™ pT7CFE1-NFtag Vector for Mammalian Cell-Free Protein Expression
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Thermo Scientific™ T7 Cell-Free Expression Vectors (pT7CFE1) are cloning plasmids optimized to use with the Thermo Scientific 1-Step Human In Vitro Protein Expression System for in vitro translation (IVT) of tagged fusion proteins.
Highlights:
Optimized performance – designed to provide the highest yield in the human in vitro translation system
Many options – multiple tag and tag-location options available
Modular MCS – multiple cloning site is maintained across vector family to facilitate subcloning
Cleavable tags – HRV 3C cleavage site available on select vectors
pT7CFE1 Plasmid Features:
- EMCV IRES at the 5-prime UTR promotes high-level translation of mRNAs
- MCS accommodates gene insertion via ten different restriction sites: Msc1, Nde1, BamH1, EcoR1, EcoRV, Pac1, Pst1, Sac1, Sal1, Not1 and Xho1
- Poly A sequence in the 3-prime region promotes mRNA stabilization and protection from nucleases
- T7 terminator ensures synthesis of accurate size mRNA transcripts
- Plasmid linearization may be accomplished with restriction sites between Poly A sequence and the T7 terminator region
Specifications
Specifications
| Description | pT7CFE1-NFtag Vector |
| Type | pT7CFE1-NFtag Vector |
| Cloning Method | Restriction Enzyme/MCS |
| Protein Tag | FLAG |
| Quantity | 10 μg |
| Content And Storage | Store between -70°C and -20°C. |
| Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
| Promoter | T7 |
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