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Thermo Scientific™ PCR Master Mix (2X)

Optimize routin PCR with this highly thermostable DNA polymerase.

Brand:  Thermo Scientific™ K0172

Additional Details : Weight : 2.44490kg

 View more versions of this product

Product Code. 11863913

  • € 408.00 / 1000 reactions


In stock, ready to ship: 10
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Description

Description

A 950 bp single copy gene from human genomic DNA was amplified at a wide range of Mg2+ concentrations.
M - GeneRuler 100 bp Plus DNA Ladder

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.

PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Highlights

  • Thermostable -half life is more than 40 min at 95°C
  • Generates PCR products with 3'-dA overhangs
  • Supplied with two buffers -10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Specifications

Specifications

1 X
Low
1000 rxns
Standard
PCR Master Mix (2X)
No
Taq DNA Polymerase
SuperMix or Master Mix
3'-A
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In certain countries use of this product is covered by patents. Purchase of product in these countries includes non-transferable, limited license for using only this amount of product for the purchaser's own internal research.