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Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent
Efficient, gentle lysis and extraction of E.coli and other bacterial cells
Marke: Thermo Scientific™ 89822
Additional Details : Weight : 0.50000kg
Includes: Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.
Beschreibung
- An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
- All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
- This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
- Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.
Features of the B-PER Bacterial Protein Extraction Reagent:
- Detergent in Tris buffer with Lysozyme and Universal Nuclease
- Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
- Recovery of large molecular weight proteins (high MW proteins)
- Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
- More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications
Spezifikation
| Store at 4°C. | |
| Proprietary detergent in 20mM Tris buffer, pH 7.5, containing lysozyme and universal nuclease | |
| Bacterial Protein Extraction Reagent |
| B-PER™ | |
| 500 mL | |
| 500 mL |
Sicherheit und Handhabung
Produktidentifikator
Signalwort
Gefahrenkategorie
Gefahrenhinweis
Sicherheitshinweis
Ergänzende Informationen
- BPER Complete Bacterial Protein Extraction Reagent
Signalwort
- Warning
Gefahrenkategorie
- Serious eye damage/eye irritation Category 2
Gefahrenhinweis
- H319-Causes serious eye irritation.
Sicherheitshinweis
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
Ergänzende Informationen
- MIXTURE LIST-Contains : Poly(oxy-1,2-ethanediyl), .alpha.-[4-(1,1,3,3-tetramethyl butyl)phenyl]-.omega.-hydroxy
For Research Use Only. Not for use in diagnostic procedures.