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  5. Invitrogen™ Rat TIMP-1-Cell Lysates ELISA Kit

Invitrogen™ Rat TIMP-1-Cell Lysates ELISA Kit

Código de producto. 18527904
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests
Tamaño de la unidad:
1 set
Each
Pack of 10
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18527904 10 x 96 Tests Pack of 10
15475393 96 Tests 1 set
17125939 5 x 96 Tests Each
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Código de producto. 18527904 Proveedor Invitrogen™ N.º de proveedor ERTIMP1CLX10

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Descripción

ELISA

The Rat Tissue Inhibitor of Matrix MetalloProteinase 1-Cell Lysates (Rt TIMP-1) ELISA quantitates Rt TIMP-1 in rat cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Rt TIMP-1. Principle of the method The Rat TIMP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.
TRUSTED_SUSTAINABILITY

Especificaciones

Accession Number P30120
Assay Range 4.1 to 3000 pg/mL
Assay Sensitivity 4 pg/mL
Calibration Range 4 pg/mL to 3000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Cytokines & Receptors
Sample Type Cell Lysates
Specificity The antibody pair provided in this kit recognizes rat TIMP-1.
Sufficient For 96 Assays
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 116510
Gene Symbol Timp1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Research Discipline Oncology
Sample Volume Cell Lysate 20 μL
Storage Requirements 2°C to 8°C
Synonym TIMP-1, Timp
Target TIMP1
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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