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TNF alpha Polyclonal Antibody, PeproTech®, Invitrogen™
Goat Polyclonal Antibody
€ 204.00 - € 1716.00
Specifications
Antigen | TNF alpha |
---|---|
Concentration | 0.1-1.0 mg/mL |
Applications | ELISA, Western Blot |
Classification | Polyclonal |
Conjugate | Unconjugated |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
17885523
|
Invitrogen
500-P31AG-50UG |
50 μg |
€ 204.00
50µg |
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17895113
|
Invitrogen
500-P31AG-100UG |
€ 257.00
100µg |
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17894843
|
Invitrogen
500-P31AG-1MG |
1 mg |
€ 1716.00
1mg |
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Description
AA Sequence of recombinant protein: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human TNF-alpha. Anti-Human TNF-alpha-specific antibody was purified by affinity chromatography employing an immobilized Human TNF-alpha matrix. Sandwich ELISA: To detect Human TNF-alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TNF-alpha (500-P31AGBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TNF-alpha. Western Blot: To detect Human TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human TNF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.Specifications
TNF alpha | |
ELISA, Western Blot | |
Unconjugated | |
Goat | |
Human | |
P01375 | |
7124 | |
E.coli-derived Recombinant Human TNF-alpha | |
-20°C |
0.1-1.0 mg/mL | |
Polyclonal | |
Lyophilized | |
RUO | |
PBS with no preservative | |
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor | |
Tnf | |
Antigen affinity chromatography | |
Tnf |