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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells
Beskrivelse
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Topoisomerase I-activated pCR™II-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water
Includes the pCR™II-TOPO TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable cloning method, featuring a 5-minute cloning reaction and 3-step protocol, and yielding up to 95% recombinants. Resistant to Kanamycin (KanR) andZeocin™ (ZeoR)
Kit Options: TOPO TA Cloning Dual Promoter Kits
TOPO TA Cloning Dual Promoter kits can be purchased with a variety of competent cells that deliver different advantages depending upon your needs:
- General cloning: TOP10 cells (Cat. Nos. K4600-J10, K4600-01, K4600-40)
- High-efficiency cloning: TOP10 Electrocomp™ cells (Cat. Nos. K4660-01, K4660-40)
- General cloning, bacteriophage T1 resistance: DH5α-T1R cells (Cat. Nos. K4620-01, K4620-40)
- Fast growth: Mach1™ -T1R chemically competent E. coli (Cat. No. K4610-20)
- Repressor/induction needs: TOP10F' cells (Cat. Nos. K4650-01, K4650-40)
- Provide your own cells (Cat. Nos. 450640 and 452640)
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- T7 and SP6 promoters for efficient in vitro transcription
- M13 forward and reverse primer sites for sequencing
- 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
- Kanamycin and ampicillin resistance for your choice of selection in E. coli
- Easy blue/white colony screening for selection of recombinants
Cloning, PCR Cloning
Tekniske data
Tekniske data
| Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
| Cloning Method | TOPO™-TA |
| Content And Storage | Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
| Format | Kit |
| For Use With (Application) | Cloning and subsequent in vitro transcription |
| Includes | Topoisomerase I-activated pCR™II-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water |
| Product Line | TOPO™ |
| Product Type | TA Cloning Kit |
| Promoter | T7, SP6 |
| Quantity | 10 Reactions |
| Vis mere |
For Research Use Only. Not for use in diagnostic procedures.
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