Invitrogen™ Urea Nitrogen (BUN) Colorimetric Detection Kit

Description

The Urea Nitrogen (BUN) Detection research-use-only kit is a colorimetric assay designed for the quantification and detection of urea nitrogen in serum, plasma, urine, saliva and tissue culture media samples.

This complete, ready-to-use kit includes clear 96-well plate(s), urea nitrogen standard, and other components to perform the assay. A 96-well microplate reader capable of reading optical absorption at 450 nm is required for use of this kit.

Performance characteristics
• Assay type: colorimetric detection kit
• Sample types: serum, plasma, urine, saliva, and tissue culture media samples
• Sensitivity: 0.030 mg/dL
• Standard curve range: 0.156–10 mg/dL
• Reactivity: species independent

Background
Urea is a by-product of protein metabolism by the liver, and is therefore removed from the blood by the kidneys. Urea freely filters through the glomerulous, but is reabsorbed by the renal tubules in a flow-dependent fashion. The higher the flow rate, the greater amount of urea nitrogen is cleared from circulation and eliminated through the kidneys. Urea nitrogen is identical across all species and this kit will measure urea nitrogen from sources other than human.

Assay principle
The Urea Nitrogen ((also called BUN) Colorimetric Detection Kit is designed to quantitatively measure urea nitrogen in a variety of samples. A urea nitrogen standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with color reagents A and B and incubated at room temperature for 30 minutes. The colored product is read at 450 nm.