Vβ4 T-Cell Receptor Rat anti-Mouse, Brilliant Violet 421, Clone: KT4, BD Optibuild™

Description

The KT4 antibody specifically recognizes the Vβ4 T-cell Receptor (TCR) of mice having the a ( e.g /., C57BR, C57L, SJL, SWR), b ( e.g /., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2), and c ( e.g /., RIII) haplotypes of the Tcrb /gene complex. Vβ4 TCR-bearing cells are among the CD4+ autoreactive T cells which induce autoimmune thyroiditis after elimination of regulatory (CD5-bright) T-cell subsets. Plate-bound KT4 antibody activates Vβ4 TCR-bearing T cells.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.