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Gibco™ Viral Production Cells 2.0
Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System.
Brand: Gibco™ A51218
This item is not returnable.
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Description
- Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line
- They are a core component of the AAV-MAX Helper-Free AAV Production System
- The cells have been adapted to the chemically defined Viral Production Medium and are maintained in suspension culture in that medium
- Viral Production Cells 2.0 are highly transfectable using the AAV-MAX Transfection Kit for adeno-associated virus (AAV) production
- Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Viral Production Medium and 10% DMSO
- Cells should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance
Features of the Viral Production Cells 2.0 include:
- Clonal, 293F-derived, high-producer cell line
- Absence of SV40 large T antigen
- Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
- Enable production of high AAV titers, greater than 5 x 1010 vg/mL (unpurified)
- Robust scalability and passage stability
Specifications
Frozen | |
6 cryovials, store in vapor phase liquid nitrogen | |
Kidney (Embryonic) | |
Frozen | |
6 vials | |
Gibco Viral Production Medium | |
AAV-MAX | |
1 mL | |
Human |
1e7 cells/mL | |
Viral Production Cells 2.0 | |
Suspension Cell Culture | |
Human | |
AAV Production | |
1 x 107 cells | |
Viral Production Cells | |
dry ice for domestic shipment; liquid nitrogen for international shipment |
For Research Use Only. Not for use in diagnostic procedures.