missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Recombinant Proteins
  4. Eurofins DiscoverX Wee1 Protein, Active

Eurofins DiscoverX Wee1 Protein, Active

Product Code. 17380794
Encompass_Preferred
Click to view available options
Quantity:
10 μg
250 μg
Unit Size:
10µg
250µg
This item is not returnable. View return policy

Product Code. 17380794

Brand: Eurofins DiscoverX 14925

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Active, N-terminal GST-tagged and C-terminal 6His tagged, recombinant, human Wee1, amino acids 214-end, for use in Kinase Assays.

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

GenBank NM_003390

Specifications

Formulation mg/mL of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Molecular Weight (g/mol) 77 kDa
Quantity 10 μg
Species Human
Recombinant Recombinant
Protein Tag N-terminal GST and C-terminal 6His
Expression System Recombinant enzyme expressed in Sf21 insect cells
Protein Form Truncated
Purity or Quality Grade ≥69%
Protein Wee1, active
Product Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.