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  5. Melford X-GAL

Melford X-GAL

Product Code. 30018093
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100mg
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Product Code. Quantity unitSize
30018093 10 g 10g
30018090 100 mg 100mg
30018094 100 g 100g
30018092 1 g 1g
30018095 5 g 5g
30018091 250 mg 250mg
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Product Code. 30018093 Supplier Melford Supplier No. B7180010.0

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Description

Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies.

Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.

Water Content: 0.05% (K. Fisher)

Protect from light and moisture

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Quantity 10 g
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