Recombinant Proteins
Recombinant Proteins
Modified or manipulated proteins encoded by recombinant DNA and suitable for a variety of purposes including the modification of gene sequences, mass protein production, and the manufacture of commercial products.
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Gibco™ Mouse Sonic Hedgehog C25II Recombinant Protein
Recombinant Sonic Hedgehog C25II is a bioactive protein intended for use in cell culture applications
Shipping Condition | Wet Ice |
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Protein Family | Growth Factors & Receptors |
Content And Storage | This product is supplied lyophilized. Store at 2 to 8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤ -20°C. Avoid repeated freeze⁄thaw cycles. |
Purity or Quality Grade | 95 % |
Form | Lyophilized |
Molecular Weight (g/mol) | 19.7 kDa |
Endotoxin Level | < 0.1 ng/μg |
Gene Symbol | SHH |
Expression System | E. coli |
For Use With (Application) | Cell Culture |
Protein Subtype | Sonic Hedgehog |
Accession Number | BC063088 |
Purification Method | Sequential Chromatography |
Gene Alias | Sonic Hedgehog C25II |
Protein Form | Recombinant, Ligand, Mutant |
Protein Length | 175 aa |
Gene ID (Entrez) | 6516263 |
Classification | Carrier-Free |
Research Category | Neurobiology, Signal Transduction, Development, Differentiation, Stem Cell Research, Oncology |
Specific Activity | 5×103 to 0.83 × 102 units⁄mg |
Species | Mouse |
Product Line | Gibco™ |
Purity or Quality Grade | ≥ 98% by SDS-PAGE gel and HPLC analyses. |
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Conjugate | Unconjugated |
Specific Reactivity | Human |
Form | Lyophilized |
Molecular Weight (g/mol) | 20 |
Gene Symbol | Shh |
Endotoxin Concentration | <1 EU/μg |
Sequence | IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG |
Storage Requirements | -20°C |
For Use With (Application) | Functional Assay |
Source | E. coli |
Name | Human Sonic Hedgehog C25II |
Accession Number | Q15465 |
Regulatory Status | RUO |
Gene Alias | 9530036O11Rik; Dsh; fc83d08; hedgehog; hemimelic extra toes; Hhg1; HHG-1; HLP3; HPE3; Hx; Hxl3; M100081; MCOPCB5; morphogen; shh; Shh N-terminal processed signaling domains; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; shh.L; shha; ShhN; ShhNC; ShhNp; short digits; SMMCI; sonic hedgehog; sonic hedgehog a; sonic hedgehog homolog; sonic hedgehog L homeolog; sonic hedgehog protein; Sonic hedgehog protein 19 kDa product; Sonic hedgehog protein A; Sonic hedgehog protein A N-product; Sonic hedgehog protein N-product; sonic hedgehog signaling molecule; sonic hedgehog signaling molecule a; sonic-you; syu; TPT; TPTPS; vhh1; vhh-1; wu:fc83d08; XELAEV_18030676mg; Xhh; xshh; X-SHH |
Gene ID (Entrez) | 6469 |
Formulation | protein with no preservative |
Recombinant | Recombinant |
Purity or Quality Grade | ≥ 90% by SDS-PAGE gel and HPLC analyses. |
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Conjugate | Unconjugated |
Specific Reactivity | Mouse |
Form | Lyophilized |
Molecular Weight (g/mol) | 20 |
Gene Symbol | Shh |
Endotoxin Concentration | <1 EU/μg |
Sequence | IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG |
Storage Requirements | -20°C |
For Use With (Application) | Functional Assay |
Source | E. coli |
Name | Mouse Sonic Hedgehog C25II |
Accession Number | Q62226 |
Regulatory Status | RUO |
Gene Alias | 9530036O11Rik; Dsh; fc83d08; hedgehog; hemimelic extra toes; Hhg1; HHG-1; HLP3; HPE3; Hx; Hxl3; M100081; MCOPCB5; morphogen; shh; Shh N-terminal processed signaling domains; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; shh.L; shha; ShhN; ShhNC; ShhNp; short digits; SMMCI; sonic hedgehog; sonic hedgehog a; sonic hedgehog homolog; sonic hedgehog L homeolog; sonic hedgehog protein; Sonic hedgehog protein 19 kDa product; Sonic hedgehog protein A; Sonic hedgehog protein A N-product; Sonic hedgehog protein N-product; sonic hedgehog signaling molecule; sonic hedgehog signaling molecule a; sonic-you; syu; TPT; TPTPS; vhh1; vhh-1; wu:fc83d08; XELAEV_18030676mg; Xhh; xshh; X-SHH |
Gene ID (Entrez) | 20423 |
Formulation | protein with no preservative |
Recombinant | Recombinant |
Buffer | 10 mM sodium phosphate, pH 7.5 |
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Purity or Quality Grade | Greater than 95% as determined by SDS-PAGE. |
Gene Symbol | SHH |
Endotoxin Concentration | Less than or equal to 1.00 EUs/ug as determined by the LAL method. |
Sequence | MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG |
Name | SHH / Sonic hedgehog Protein |
Accession Number | NP_000184.1 |
Regulatory Status | Research Use Only |
Product Type | Recombinant Protein |
Biological Activity | Shows bioactivity at a concentration of 5 ug/mL in the CCL-226 cell proliferation assay. The bioactivity acceptance criteria is set at 200 units/mg. |
Gene ID (Entrez) | 6469 |
Cross Reactivity | Human |
Protein Tag | Untagged |
Species | E. coli |
Buffer | 10 mM sodium phosphate, pH 7.5 |
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Purity or Quality Grade | Greater than 95% as determined by SDS-PAGE. |
Gene Symbol | SHH |
Endotoxin Concentration | Less than or equal to 1.00 EUs/ug as determined by the LAL method. |
Sequence | MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG |
Name | SHH / Sonic hedgehog Protein |
Accession Number | NP_033196.1 |
Regulatory Status | Research Use Only |
Product Type | Recombinant Protein |
Biological Activity | Sonic Hedgehog ( SHH ) activity was confirmed in a CCL-226 cell proliferatin assay. See figure for results. |
Gene ID (Entrez) | 20423 |
Cross Reactivity | Mouse |
Protein Tag | Untagged |
Species | E. coli |
Proteintech™ HumanKine™ Recombinant Human Sonic Hedgehog (SHH) Protein
HumanKine™ cytokines and growth factors are human cell expressed proteins in HEK293 cells
Content And Storage | Lyophilized proteins are stable for 1 year from the date of receipt if stored between -20 to -80°C. Upon reconstitution, it is recommended the solution be stored at 4°C for short term or at -20 to -80°C for long term. Repeated freeze thaw cycles should be avoided with reconstituted products. |
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Purity or Quality Grade | > 90% |
Form | Lyophilized |
Molecular Weight (g/mol) | 20 to 24 kDa |
Gene ID (Entrez) | 6469 |
Activity | Typically ≤ 350 ng/mL EC50 |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
Expression System | Human embryonic kidney 293 (HEK293) human cell expression system |
Species | Human |
ACROBiosystems Human Sonic Hedgehog / Shh Protein, His Tag
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Buffer | PBS, pH7.4 |
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Purity or Quality Grade | >95% as determined by SDS-PAGE |
Conjugate | Unconjugated |
Form | Powder |
Common Name | Sonic Hedgehog |
Molecular Weight (g/mol) | 20.4 kDa |
Endotoxin Level | Less than 1.0 EU / μg by the LAL method. |
Storage Requirements | -20°C |
Expression System | HEK293 |
Source | Human Sonic Hedgehog, His Tag (SH7-H5229) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Gly 197 (Accession # NP_000184). |
Protein Subtype | Cytokines and Growth Factors |
Accession Number | NP_000184 |
Gene Alias | SHH,HHG-1,Sonic hedgehog,HLP3,HPE3,SMMCI,TPT,TPTPS |
Immunogen | Cys 24 - Gly 197 |
Protein Tag | His Tag |
Species | Human |
Purity or Quality Grade | ≥ 95% by SDS-PAGE gel and HPLC analyses. |
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Conjugate | Unconjugated |
Specific Reactivity | Mouse |
Form | Lyophilized |
Molecular Weight (g/mol) | 23.3 |
Gene Symbol | FGF9 |
Endotoxin Concentration | <1 EU/μg |
Sequence | PLGEVGSYFG VQDAVPFGNV PVLPVDSPVL LNDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS |
Storage Requirements | -20°C |
For Use With (Application) | ELISA,Functional Assay,Western Blot |
Source | E. coli |
Name | Mouse FGF-9 |
Accession Number | P54130 |
Regulatory Status | RUO |
Gene Alias | Eks; elbow knee synostosis; FGF; Fgf9; FGF-9; Fibroblast growth factor; Fibroblast growth factor 9; fibroblast growth factor 9 (glia-activating factor); GAF; glia activating factor; glia-activating factor; HBFG-9; HBGF-9; Heparin-binding growth factor 9; M-FGF-9; SYNS3 |
Gene ID (Entrez) | 14180 |
Formulation | protein with no preservative |
Recombinant | Recombinant |
Purity or Quality Grade | ≥ 95% by SDS-PAGE gel and HPLC analyses. |
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Conjugate | Unconjugated |
Specific Reactivity | Human |
Form | Lyophilized |
Molecular Weight (g/mol) | 23.2 |
Gene Symbol | FGF9 |
Endotoxin Concentration | <1 EU/μg |
Sequence | PLGEVGNYFG VQDAVPFGNV PVLPVDSPVL LSDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS |
Storage Requirements | -20°C |
For Use With (Application) | Functional Assay |
Source | E. coli |
Name | Human FGF-9 |
Accession Number | P31371 |
Regulatory Status | RUO |
Gene Alias | Eks; elbow knee synostosis; FGF; Fgf9; FGF-9; Fibroblast growth factor; Fibroblast growth factor 9; fibroblast growth factor 9 (glia-activating factor); GAF; glia activating factor; glia-activating factor; HBFG-9; HBGF-9; Heparin-binding growth factor 9; M-FGF-9; SYNS3 |
Gene ID (Entrez) | 2254 |
Formulation | protein with no preservative |
Recombinant | Recombinant |
Purity or Quality Grade | ≥ 95% by SDS-PAGE gel and HPLC analyses. |
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Conjugate | Unconjugated |
Specific Reactivity | Human |
Form | Lyophilized |
Molecular Weight (g/mol) | 23.2 |
Gene Symbol | FGF9 |
Endotoxin Concentration | <0.1 EU/μg |
Sequence | PLGEVGNYFG VQDAVPFGNV PVLPVDSPVL LSDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS |
Storage Requirements | -20°C |
For Use With (Application) | Functional Assay |
Source | E. coli |
Name | Human FGF-9, Animal-Free |
Accession Number | P31371 |
Regulatory Status | RUO |
Gene Alias | Eks; elbow knee synostosis; FGF; Fgf9; FGF-9; Fibroblast growth factor; Fibroblast growth factor 9; fibroblast growth factor 9 (glia-activating factor); GAF; glia activating factor; glia-activating factor; HBFG-9; HBGF-9; Heparin-binding growth factor 9; M-FGF-9; SYNS3 |
Gene ID (Entrez) | 2254 |
Formulation | protein with no preservative |
Recombinant | Recombinant |
Purity or Quality Grade | ≥ 95% by SDS-PAGE gel and HPLC analyses. |
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Conjugate | Unconjugated |
Specific Reactivity | Human |
Molecular Weight (g/mol) | 23.2 kDa |
Gene Symbol | FGF9 |
Activity | Determined by its ability to stimulate the proliferation of mouse balb/c 3T3 cells using a concentration range of 1.0-5.0 ng/ml |
Endotoxin Concentration | <0.1 EU/ μg |
Sequence | PLGEVGNYFG VQDAVPFGNV PVLPVDSPVL LSDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS |
Storage Requirements | -20°C |
Expression System | E. coli |
For Use With (Application) | Functional Assay |
Name | FGF9 |
Accession Number | P31371 |
Regulatory Status | RUO |
Purification Method | HPLC, SDS-PAGE |
Gene Alias | Eks; elbow knee synostosis; FGF; Fgf9; FGF-9; Fibroblast growth factor; Fibroblast growth factor 9; fibroblast growth factor 9 (glia-activating factor); GAF; glia activating factor; glia-activating factor; HBFG-9; HBGF-9; Heparin-binding growth factor 9; M-FGF-9; SYNS3 |
Format | Lyophilized |
Product Type | Protein |
Gene ID (Entrez) | 2254 |
Formulation | protein with no preservative |
Recombinant | Recombinant |
Purity or Quality Grade | ≥ 95% by SDS-PAGE gel and HPLC analyses. |
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Conjugate | Unconjugated |
Specific Reactivity | Human |
Molecular Weight (g/mol) | 19.7 kDa |
Gene Symbol | Fgf4 |
Activity | Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 10 ng/mL, corresponding to a specific activity of ≥ 1 x 10^5 units/mg. |
Endotoxin Concentration | <0.1 EU/μg |
Sequence | GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL |
Storage Requirements | -20°C |
Expression System | E. coli |
For Use With (Application) | Functional Assay |
Name | Fgf4 |
Accession Number | P08620 |
Regulatory Status | RUO |
Purification Method | HPLC, SDS-PAGE |
Gene Alias | FGF; FGF4; Fgf-4; Fgfk; Fibroblast growth factor; fibroblast growth factor 4; fibroblast growth factor 4 splice isoform; HBGF-4; heparin secretory transforming protein 1; heparin secretory-transforming protein 1; heparin-binding growth factor 1; heparin-binding growth factor 4; HST; Hst1; hst-1; HSTF1; HSTF-1; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; Kfgf; K-FGF; K-fibroblast growth factor; KS3; oncogene HST; transforming protein KS3 |
Format | Lyophilized |
Product Type | Protein |
Gene ID (Entrez) | 2249 |
Formulation | Protein with no preservative |
Recombinant | Recombinant |
Purity or Quality Grade | ≥ 95% by SDS-PAGE gel and HPLC analyses. |
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Conjugate | Unconjugated |
Specific Reactivity | Human |
Form | Lyophilized |
Molecular Weight (g/mol) | 19.7 |
Gene Symbol | Fgf4 |
Endotoxin Concentration | <0.1 EU/μg |
Sequence | GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL |
Storage Requirements | -20°C |
For Use With (Application) | Functional Assay |
Source | E. coli |
Name | Human FGF-4, Animal-Free |
Accession Number | P08620 |
Regulatory Status | RUO |
Gene Alias | FGF; FGF4; Fgf-4; Fgfk; Fibroblast growth factor; fibroblast growth factor 4; fibroblast growth factor 4 splice isoform; HBGF-4; heparin secretory transforming protein 1; heparin secretory-transforming protein 1; heparin-binding growth factor 1; heparin-binding growth factor 4; HST; Hst1; hst-1; HSTF1; HSTF-1; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; Kfgf; K-FGF; K-fibroblast growth factor; KS3; oncogene HST; transforming protein KS3 |
Gene ID (Entrez) | 2249 |
Formulation | protein with no preservative |
Recombinant | Recombinant |
Purity or Quality Grade | ≥ 95% by SDS-PAGE gel and HPLC analyses. |
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Conjugate | Unconjugated |
Specific Reactivity | Human |
Form | Lyophilized |
Molecular Weight (g/mol) | 19.7 |
Gene Symbol | Fgf4 |
Endotoxin Concentration | <1 EU/μg |
Sequence | GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL |
Storage Requirements | -20°C |
For Use With (Application) | ELISA,Functional Assay,Western Blot |
Source | E. coli |
Name | Human FGF-4 |
Accession Number | P08620 |
Regulatory Status | RUO |
Gene Alias | FGF; FGF4; Fgf-4; Fgfk; Fibroblast growth factor; fibroblast growth factor 4; fibroblast growth factor 4 splice isoform; HBGF-4; heparin secretory transforming protein 1; heparin secretory-transforming protein 1; heparin-binding growth factor 1; heparin-binding growth factor 4; HST; Hst1; hst-1; HSTF1; HSTF-1; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; Kfgf; K-FGF; K-fibroblast growth factor; KS3; oncogene HST; transforming protein KS3 |
Gene ID (Entrez) | 2249 |
Formulation | protein with no preservative |
Recombinant | Recombinant |
Purity or Quality Grade | ≥ 95% by SDS-PAGE gel and HPLC analyses. |
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Conjugate | Unconjugated |
Specific Reactivity | Mouse |
Form | Lyophilized |
Molecular Weight (g/mol) | 19 |
Gene Symbol | Fgf4 |
Endotoxin Concentration | <1 EU/μg |
Sequence | APNGTRHAEL GHGWDGLVAR SLARLPVAAQ PPQAAVRSGA GDYLLGLKRL RRLYCNVGIG FHLQVLPDGR IGGVHADTRD SLLELSPVQR GVVSIFGVAS RFFVAMSSRG KLFGVPFFTD ECKFKEILLP NNYNAYESYA YPGMFMALSK NGRTKKGNRV SPTMKVTHFL PRL |
Storage Requirements | -20°C |
For Use With (Application) | Functional Assay |
Source | E. coli |
Name | Mouse FGF-4 |
Accession Number | P11403 |
Regulatory Status | RUO |
Gene Alias | FGF; FGF4; Fgf-4; Fgfk; Fibroblast growth factor; fibroblast growth factor 4; fibroblast growth factor 4 splice isoform; HBGF-4; heparin secretory transforming protein 1; heparin secretory-transforming protein 1; heparin-binding growth factor 1; heparin-binding growth factor 4; HST; Hst1; hst-1; HSTF1; HSTF-1; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; Kfgf; K-FGF; K-fibroblast growth factor; KS3; oncogene HST; transforming protein KS3 |
Gene ID (Entrez) | 14175 |
Formulation | protein with no preservative |
Recombinant | Recombinant |