PCR Equipment and Supplies
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Applied Biosystems™ TaqMan™ Universal PCR Master Mix
TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.
| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Product Type | Universal PCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
| PCR Method | qPCR |
| Product Line | TaqMan |
| Detection Method | Primer-probe |
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Kit |
| Reverse Transcriptase | SuperScript IV |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Quantity | Each |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ dNTP Set (100 mM)
Invitrogen 100 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water. Each nucleotide is at a concentration of 100 mM.
| Shipping Condition | Approved for shipment on Wet or Dry Ice |
|---|---|
| Form | Liquid |
| Label or Dye | Unlabeled |
| Concentration | 100 mM |
| For Use With (Application) | Standard PCR,RT-PCR,Real Time PCR (qPCR) |
Thermo Scientific™ Exo-Resistant Random Primer
Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions.
Invitrogen™ Platinum™ SuperFi II PCR Master Mixes
Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Standard PCR,High-fidelity PCR |
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
| Shipping Condition | Wet Ice |
|---|---|
| InhibitorToleranceSimple | Medium |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Multiplex Capability | Single-plex |
| Detection Method | SYBR |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Polymerase | Dual-Lock Taq DNA Polymerase |
| Format | Tube |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System |
| Sample Type | DNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | cDNA or gDNA only |
| Product Line | PowerUP, SYBR |
TaqMan™ Advanced miRNA cDNA Synthesis Kit
Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.
Applied Biosystems™ QuantStudio™ 3/5 Spectral Calibration Plate 1 (FAM™, VIC™, ROX™, SYBR™ dyes), 96-well, 0.1 mL
Plate establishes the pure dye spectra and multicomponent values for FAM™, VIC™, ROX™, and SYBR™ dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.1mL RT-PCR systems.
Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)
Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.
| Polymerase | DreamTaq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Reaction Speed | Standard |
| Hot Start | No |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR
PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | • PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark. |
| InhibitorToleranceSimple | High |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Microbial Detection |
| PCR Method | qPCR |
| Multiplex Capability | Single-plex |
| Detection Method | SYBR |
| Includes | dUTP, UNG/UDG |
| Thermostability | 8 hr., Assembled Reaction |
| Polymerase | Taq DNA Polymerase |
| Format | Tube |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000 |
| Sample Type | DNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | cDNA or gDNA only |
| Product Line | Applied Biosystems |
Thermo Scientific™ Phusion™ Plus PCR Master Mixes
Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Ready to use master mix |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Platinum™ SuperFi™ DNA Polymerase
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Second Strand cDNA Synthesis Kit
Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
| Reaction Format | Separate Components |
|---|---|
| Final Product Type | Double Stranded cDNA |
| Product Type | cDNA Synthesis Kit |
| For Use With (Application) | Cloning |
Thermo Scientific™ Phusion™ Plus DNA Polymerase
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.
| Shipping Condition | Room Temp or Wet Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate components |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |