Real Time PCR Master Mixes

Real-time PCR master mixes are batch mixtures of PCR reagents at optimal concentrations that can be prepared and divided among multiple PCR tubes/plates. They reduce pipetting and risk of contamination, save time, and limit possible mixing errors.

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Applied Biosystems™ TaqMan™ Gene Expression Master Mix

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.

Pricing and Availability
Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
Purity or Quality Grade	UP (Ultra Pure)
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	TaqMan
Detection Method	Primer-probe

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Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

Pricing and Availability
Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Product Line	TaqMan
Detection Method	Primer-probe

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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Multiplex Capability	Single-plex
Detection Method	SYBR
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Dual-Lock Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System
Sample Type	DNA
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	Medium
Target Specificity	cDNA or gDNA only
Product Line	PowerUP, SYBR

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Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Product Type	Universal Master Mix II
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Product Line	TaqMan

Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR

TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Reverse Transcriptase	M-MLV
Form	Liquid
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 3-plex
Detection Method	Primer-probe
Thermostability	Not Applicable
Polymerase	AmpliTaq Fast DNA Polymerase
Format	Tube
Product Type	1-Step Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System
Sample Type	Total RNA, Viral DNA, Viral RNA, mRNA
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	High
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Built-In Hot Start
Product Line	TaqMan

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Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq DNA Polymerase
Format	Tube
Product Type	Fast Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast
Product Line	AmpErase, TaqMan

Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix

TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Concentration	4X
For Use With (Application)	Gene Expression,Microbial Detection
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 4-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	Not Applicable
Polymerase	Fast DNA Polymerase
Sensitivity	Down to 10 copies
Format	Tube
Product Type	qPCR Master Mix
Passive Reference Dye	Mustang Purple
For Use With (Equipment)	QuantStudio 6 Pro Real-Time PCR System or QuantStudio 7 Pro, QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7, 7500 , 7500 Fast, 7500 Fast Dx
Sample Type	DNA, RNA
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	High
Target Specificity	RNA only, Both RNA and DNA
Product Line	TaqPath

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Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Pricing and Availability
Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
Reaction Speed	Standard
Label or Dye	SYBR Green
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	SYBR
Detection Method	SYBR

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Applied Biosystems™ SYBR™ Select Master Mix

Cost-effective solution for real-time PCR applications.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Purity or Quality Grade	UP (Ultra Pure)
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq DNA Polymerase
Format	Vial
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast or Standard
Label or Dye	SYBR GreenER
Hot Start	Built-In Hot Start
Sufficient For	100 Reactions of 20 μL
Product Line	SYBR

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Applied Biosystems™ Power SYBR™ Green PCR Master Mix

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Purity or Quality Grade	LD (Low DNA)
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Label or Dye	SYBR Green
Product Line	SYBR

Applied Biosystems™ Path-ID™ qPCR Master Mix

Ambion™ Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan™ probe-based, real-time PCR detection of DNA targets.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	2X qPCR Master Mix is stored at –20°C. Nuclease-free water may be stored at any temperature.
Purity or Quality Grade	UP (Ultra Pure)
Form	Liquid
For Use With (Application)	Animal Health,Pathogen Detection
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Format	Tube
Passive Reference Dye	ROX (Pre-mixed)
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast or Standard
Hot Start	Built-In Hot Start
Product Line	Ambion, Path-ID

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	Medium
Content And Storage	Store at -15°C to -25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form	Liquid
Concentration	4X
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 5-plex
Detection Method	Primer-probe
Includes	dUTP
Thermostability	8 hr., Assembled Reaction
Polymerase	Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format	Single-Tube
Passive Reference Dye	ROX (Pre-mixed)
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	High
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Antibody-based
Shelf Life	Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line	TaqPath

Applied Biosystems™ VetMAX™-Plus qPCR Master Mix

For use with VetMAX™ Reagents and VetMAX™ Controls.

Pricing and Availability

Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG

Is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2).

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Product Type	Quantitative PCR SuperMix-UDG
For Use With (Equipment)	7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Platinum
Detection Method	Primer-probe

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Applied Biosystems™ TaqPath™ qPCR Master Mix, CG

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

Pricing and Availability
Specifications

Content And Storage	Store at 2°C to 8°C in the dark.
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression,Microbial Detection,DNA Quantification,Presence/Absence,miRNA analysis
PCR Method	qPCR
Multiplex Capability	Duplex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	48 hr., Assembled Reaction
Polymerase	Fast DNA Polymerase
Sensitivity	Down to 10 copies
Format	Bottle
Product Type	qPCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	QuantStudio Dx, 7500 Fast Dx, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System
Sample Type	cDNA, DNA
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	High
Target Specificity	cDNA or gDNA only
Hot Start	Built-In Hot Start
Product Line	TaqPath

Real Time PCR Master Mixes

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Applied Biosystems™ TaqPath™ qPCR Master Mix, CG Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Applied Biosystems™ TaqPath™ qPCR Master Mix, CG